Table 1 Primers used for detection of iscS genes and genes in dnd operon
Primer | Oligonucleotide sequence (5' to 3') | Target gene | Amplicon size (bp) | Annealing temperature (°C) | Reference(s) |
|---|---|---|---|---|---|
IscS-CMu F | ATCTGACAACCTGGCGATCA | iscS | 860 | 44 | An et al., 2012 [11] |
IscS-CMu R | CTTCAGTAGTAAAACGACCT | ||||
dndC-F | CCGTATCGGTCGCATTGTT | dndC | 579 | 51 | Wang et al, 2011 [15] |
dndC-R | CTTCGTCCCATTTCAGGTT | ||||
dndB-F | CATTGCCAGATAACGCGCC | dndB | 207 | 50 | This study |
dndB-R | CGTAAAGCGATTGAAGACGC | ||||
dndC-F | TTTCCACCCCACGGGCTTTC | dndC | 532 | 56 | This study |
dndC-R | GCCTGCCCCCGGAAATG | ||||
dndD-F | AGCCTCCTT(A/T)GTTAATTCCCAGA | dndD | 605 | 51 | This study |
dndD-R | AGCAACTGGA(T/C)TGTGT(C/T)CGT | ||||
dndE-F | GC(A/G)AT(C/T)CCATCCTC(C/A)ACAT | dndE | 196 | 48 | This study |
dndE-R | GCTCGGTGGAGAGTGAGT |