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Table 2 Primers used to determine the immediate genetic environment of dnd operons of E. coli strains

From: The dnd operon for DNA phosphorothioation modification system in Escherichia coli is located in diverse genomic islands

Primer

Oligonucleotide sequence (5' to 3')

Target gene

Amplicon size (bp)

Annealing temperature (°C)

Reference(s)

gp_a_US-F

AACAACTGTGGGTCAGCGAA

upstream of

489

52

this study

gp_a_US-R

AGGCTATCTGATGCTCCCGA

dnd operon

gp_b_US-F

TGGGGGTTGAGTCTGATGAT

upstream of

723

52

this study

gp_b_US-R

TCGAATGGTGCTGAGTCGTC

dnd operon

gp_cI_US-F

GAAACCAACCCTCTTTTCACGTC

upstream of

823

52

this study

gp_cI_US-R

GTCTGATGCTCCCGAATCGAA

dnd operon

gp_cII_US-F

ACCATCGAAAGCCCCATTAAGAG

upstream of

760

52

this study

gp_cII_US-R

GCTGTCTGATGCTCCCGAAT

dnd operon

gp_d_US-F

AGGCTGGAAGCCATGTTTTG

upstream of

905

52

this study

gp_d_US-R

TTCGTCGATCAACTGCGTGA

dnd operon

gp_e_US-F

CCATATGTCAGCTCAAGTCGC

upstream of

612

52

this study

gp_e_US-R

TACTGATACGGAAGTGGATGAGC

dnd operon

gp_f_US-F

GGGCGAGTTCGATGCTATGAC

upstream of

595

53

this study

gp_f_US-R

TGGGGGAGACACTACAAGCTA

dnd operon

gp_a_DS-F

ATTCGTGCCGGGAAACTCAT

downstream of

636

52

this study

gp_a_DS-R

TCAGTGCTGTGCGTAGTGAG

dnd operon

gp_b_DS-F

CGCTTTACGACGATGCTGAC

downstream of

591

52

this study

gp_b_DS-R

GCGAAACCAAGACGTGG(A/T)CT

dnd operon

gp_c_DS-F

CGTTGAGCGCGTAATTCTGG

downstream of

582

52

this study

gp_c_DS-R

ATCAGGGGCTTCTTGCAGAC

dnd operon

gp_d_DS-F

CGCGTCCATCGGTACACATA

downstream of

558

52

this study

gp_d_DS-R

TGCCACTTCAGTGCTGACAA

dnd operon

gp_e_DS-F

AACGGCAATAGACGCTGTCA

downstream of

518

52

this study

gp_e_DS-R

AGCAACCGTCTTCGTTCTGT

dnd operon

gp_f_DS-F

ACATCTGCTGGCTACGCTTT

downstream of

715

52

this study

gp_f_DS-R

TTGTCATGCGGTCTTAGCGA

dnd operon