Figure 3

Digital gene expression of myogenic related genes in pacu fast and slow skeletal muscle. (A) Myogenic related genes represented in the transcriptome mapped into a schematic reconstruction of the myogenesis process. “Red circles” and “Empty circles” indicate components significantly higher in slow and fast skeletal muscle respectively muscle (FDR < 0.05). “Green circles” indicate components with no significant differences between fibre types. (B) Myogenic regulatory factors (MRFs) digital gene expression. (C) Myocyte-specific enhancer factor 2 genes digital gene expression. (D) Myostatin digital gene expression. (E) Calpain 3 and cysteine rich domain 3 digital gene expression. Digital gene expression is represented using a logarithmic scale for slow (full bars) and fast (empty bars) skeletal muscle. Significant differences for paralogues within (***; FDR < 0.001) and between (#; FDR < 0.05) fibre types are indicated. Values represent mean ± SE (n = 5). Myod1: myoblast determination protein 1; myf5: myogenic factor 5; mrf4: myogenic regulatory factor 4; myog: myogenin; mef2: myocyte specific enhancer factor 2; capn3: calpain-3; stac3: cysteine rich domain 3; mstn: myostatin.