Figure 4

Validation of DEGs by qPCR. Twelve DEGs were random selected for qPCR analysis and compared with the equivalent RNA-seq data. The data from qPCR were presented as mean ± standard deviation of three replicates. The data from RNA-seq were the read counts that normalized by DEseq from three replicates. A and B, Expression level of MIF and PRDX3 in comparison E-1-M/C-1-M (grey bars, C-1-M; black bars, E-1-M); C and D, APOEb and EF-1α in comparison E-1-L/C-1-L (grey bars, C-1-L; black bars, E-1-L); E and F, APOA-I-1 and PAIP2B in comparison E-2-M/C-2-M (grey bars, C-2-M; black bars, E-2-M); G and H, PSD2 and ALDOb in E-2-L/C-2-L (grey bars, C-2-L; black bars, E-2-L); I and J, ALDOa and CFD in E-2-M/E-1-M (grey bars, E-1-M; black bars, E-2-M); K and L, EFF1α1L2 and GAPDH in E-2-L/E-1-L (grey bars, E-1-L; black bars, E-2-L).