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Figure 3 | BMC Genomics

Figure 3

From: One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT)

Figure 3

PITT using TOKOM-3 seed mice. (A) Schematic diagram of the PITT injection experiment. Donor vector containing DOI is injected into the fertilized eggs harboring landing pad together with iCre and/or PhiC31o mRNA. (B) Successful targeted transgenesis in blastocysts derived from zygotes injected with pBER + iCre mRNA + PhiC31o mRNA. (C) Successful targeted transgenesis in day 13.5 fetuses. Zygotes/fetuses exhibiting red fluorescence indicate successful targeted insertion of DOI. (D, E) The PITT results in blastocysts (D) and fetuses/pups (E).

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BMC Genomics

ISSN: 1471-2164

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