Figure 6

Quantitative RT-PCR analysis of Dcr-2 , Ago-2 and PIWI -class transcripts in An. gambiae . (A-B) Transcript expression profiling in adult somatic and germline organs and tissues. Transcript levels were normalized to Rpl19 (endogenous control) and shown as a fold change above the level in one-two day old female (A) and male (B) whole body respectively. (C) Analysis of Dcr-2, Ago-2 and PIWI-class transcripts 3 h after infectious feeding and 24 h after regular blood feeding in whole female body. The transcript levels are shown as a fold change above the level in sugar-fed females. (D) Developmental profiling showing the transcript levels in embryos (emb), 1^st instar larvae (L1), 2^nd-3^rd instar larvae (L2-3), 4^th instar larvae (L4), male and female pupae (P), one-two day old male adults. Transcript levels were normalized to Rpl19 and shown as a fold change above the level in one-two day old female whole body.