De novo sequencing of the Hypericum perforatum L. flower transcriptome to identify potential genes that are related to plant reproduction sensu lato

Table 4 Diversity statistics for the unigenes aligning to the Hypericum BAC clone HM06166

Gene product	Accession	Unigenes	S/AL	Pi/AL	S/U	Pi/U	Hn/ALU
Predicted protein	XP_002883670.1	isotig08628			7	0.007	4
		isotig08629
		isotig08630
WRKY-like protein	ADK92866.1	isotig10799	4	0.000	16	0.010	3
WRKY-like protein	ADK92866.1	isotig10800	11	0.010	16	0.010	3
DGCR-like protein	ADK92867.1	isotig04455	9	0.010	21	0.022	3
DGCR-like protein	ADK92867.1	isotig04458	24	0.030	21	0.022	3
ARI7-like	XP_002310443.1	isotig26043	98	0.160	139	0.081	4
		isotig07115	126	0.170
		isotig07116	121	0.160
		isotig07117	119	0.150
Thymidine kinase (TK)	ADK92870.1	isotig09624	7	0.010	7	0.008	3
Thymidine kinase (TK)	ADK92870.1	isotig09625	7	0.010	7	0.008	3
HnRNP	ADK92872.1	isotig31453	2	0.020	26	0.037	3
		isotig11295	3	0.000
		isotig11296	20	0.030
RimL/acetyltransferase-domain protein	ADK92874.1	isotig13677	4	0.010	0	0.000	2
RimL/acetyltransferase-domain protein	ADK92874.1	isotig13678	0	0.000	0	0.000	2
Mo25-like protein	ADK92876.2	isotig05993	34	0.030	43	0.031	3
Mo25-like protein	ADK92876.2	isotig05994	36	0.030	43	0.031	3
Polygalacturonase (PG)	ADK92878.1	isotig11051	3	0.000	17	0.012	3
Polygalacturonase (PG)	ADK92878.1	isotig11052	11	0.010	17	0.012	3
ITS (nuclear)**		contig00041	1	0.002	n.a.	n.a.	2

Statistics are based on multiple unigenes aligning to unique coding sequences predicted from the BAC clone HM061166, containing the HAPPY locus associated to Apospory (AL). For each coding region (Gene product), the Unigene Id, the number of polymorphic sites (S/AL) and measured nucleotide diversity (Pi/AL) are reported. The number of polymorphic sites (S/U) and nucleotide diversity (Pi/U) calculated among unigenes are also indicated. The number of haplotypes emerging from the alignment of the multiple unigenes to coding sequences predicted from the BAC clone HM061166 is indicated as Hn/ALU. **Locus not included in the BAC clone HM061166, which was used as external reference for statistics on sequence genetic diversity. Unigenes were deposited as TSA project under the accession GBXG00000000.

ISSN: 1471-2164