Figure 2

Zygospore production of RNAi mutants. A. Mating assays of the wild type strain NRRL3631 (+) with the (−) strains R7B (wild type, left) and rdrp-2Δ (right). Sexual interaction with formation of zygospores in the contacting zone (red arrow) can be only seen in the wild type cross. A histological section of the sexual interaction area observed under optical microscopy (10X) is shown. B. Relative zygospore production in genetic crosses between the indicated (−) strains and the wild-type NRRL3631 (+). Data are the averages and standard errors of the zygospores quantified in twelve histological sections (0.003 cm²) of the sexual interaction area for each cross after normalization against the zygospores produced in crosses between wild-type strains.