Figure 5

Accumulation of mRNAs in wild type and mutant strains. Northern blot analysis of total RNA (50 μg) isolated from the wild-type strain R7B and the indicated RNAi mutants grown for 24 h and 48 h in solid rich medium (YPG) pH 4.5. Membranes were hybridized with specific probes corresponding to the indicated genes (Additional file 3: Table S3) and reprobed with an 18S rRNA probe to check loading. Color bars depicted below each northern-blot show the log₂ fold change in mutants versus wild-type strain at each time estimated from densitometric analysis of hybridization signals (upper bar) and RNA-seq analysis (lower bar).