Figure 1

A panel of read-throughs is expressed in RCC. (A) The fraction of different classes of RNA chimeras called by FusionSeq from the RNASeq data in seven frozen RCC samples. The clinical characteristics of the samples are given elsewhere [30]. ccRCC, clear cell RCC; chrRCC, chromophobe RCC; tRCC, Xp11 translocation RCC. (B) 27 read-throughs which were chosen for further validation by reverse transcription (RT)-PCR. Plotted are the individual RESPER scores per sample and the mean RESPER with range if the read-through was found in more than 1 sample. (C) Quantitative evaluation of 12 read-throughs by TaqMan assays in frozen tissue of RCC and matched adjacent normal kidney, plotted as Tukey boxplots. No read-through has significant differential expression between tumor and normal tissue as calculated by student’s t-test with correction for multiple testing by Bonferroni method. The blue outlier dot in TMED6-COG8 is the tRCC whose detailed analysis is featured elsewhere [30].