Figure 6

Functional consequences of KLK4-KRSP1 and BC039389-GATM read-throughs on gene expression. (A, B) Heatmaps showing significantly dysregulated genes by BGv1 (A) or KKv1 (B) from genome-wide expression analysis of cancer (Ca) cell lines Caki-2, ACHN, A704, LNCaP, sorted from most down-regulated to most up-regulated in read-through knock-down vs. mock treatment. Pink rectangles mark the genes chosen for TaqMan qPCR validation of the data sets (see also Additional file 1: Figures S4 and S5). Arrows indicate the genes chosen for further experimentation. (C) IL8 expression in BG and GATMwt knock-down cells compared to si negative control (si nc). (D) IL8 levels measured by ELISA in BG and GATMwt knock-down cells compared to si nc. (E) LITAF expression in KKv1 and KLK4wt knock-down cells. (F) LITAF levels on Western Blot in KKv1 and KLK4wt knock-down cells compared to si nc. Significance was calculated using student’s t-test (*p < 0.05, ns = not significant).