Figure 2

Relative gene expression. Microarray data were analysed for selected genes known for their expression in morula (purple), blastocyst (green), ICM (orange) and TE (blue) (A). The same genes were analysed by qRT-PCR in 4–6 samples per embryo stage or cell type revealing the same pattern (B). Normalization was performed with the reference genes encoding RPL15, SDHA and YWHAZ. Y-axis depicts relative mean expression to sample with highest expression set at 1 per gene. Bars with different letters are significantly different (p < 0.05) and error bars indicate standard deviation.