Skip to main content
Figure 5 | BMC Genomics

Figure 5

From: A mRNA landscape of bovine embryos after standard and MAPK-inhibited culture conditions: a comparative analysis

Figure 5

Expression in and GO-analysis of MAPK-inhibited and control inner cell mass. All probes representing genes (grey) were plotted for their expression levels (A). Black lines represent the 2-fold cut-off. Axes are Log10 transformed and depict the normalized expression signal in the indicated sample. Genes discussed in text (and analysed by qRT-PCR) are indicated. Genes with significant (p ≤ 0.05) higher expression levels in MAPK-inhibited and control inner cell masses are indicated with red and green dots, respectively. MAPK-inhibited ICM (red) gene expression of NANOG, POU5F1, SOX2 and GATA6 were determined by microarray (B) and qRT-PCR (C) presented relative to gene expression in control ICM (green). Significant differences are indicated; * p < 0.05; ** p < 0.005. IFNT expression (D) was determined by qRT-PCR in control TE (light blue), PD0325901-treated TE (dark blue) and PD0325901-treated ICM (red) and presented relative to control ICM (green). Normalization was performed with the reference genes RPL15, SDHA and YWHAZ. Error bars indicate standard deviation and data bars with different letters are significantly different (p < 0.05). A Venn diagram (E) shows the numbers of overrepresented (red; total 279) and underrepresented (green; 318 in total) GO-terms in the MAPK-inhibited inner cell mass. 189 GO-terms are in common.

Back to article page