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Figure 1 | BMC Genomics

Figure 1

From: Distinguishing low frequency mutations from RT-PCR and sequence errors in viral deep sequencing data

Figure 1

RNA virus sequencing and error introduction. The typical steps involved in processing an RNA virus sample for sequencing. At each step, errors are introduced in the form of artefactual mutations that will be present in the resultant NGS reads. Artefactual mismatches to the reference genome are practically indistinguishable from low-frequency real biological variation within the reads.

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