Figure 8
From: Distinguishing low frequency mutations from RT-PCR and sequence errors in viral deep sequencing data
Simulated number of mismatches from sequence and process error alone at a specified site. For a given site, the model simulated 4,500 replicates, with each replicate having the same coverage (25,000) and average sequence error (0.000223) of the site, but receiving a random draw from the enzyme error distributions. The simulated number of mismatches for all replicates is presented as a histogram (black bars) that can then be compared to the real number of mismatches observed at the specified site. If one had observed 15 mismatches (grey bar) in the real biological data set, it is unlikely that any of these are real given the simulated distribution. However, if one observed 30 mismatches (grey dotted bar) it is much more likely that there is real biological variation at this site.