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Table 1 Variant frequency thresholds

From: Distinguishing low frequency mutations from RT-PCR and sequence errors in viral deep sequencing data

 

25%

50%

75%

95%

100%

Seq

0.0119%

0.0183%

0.0268%

0.0466%

0.276%

PCR-Low

0.0261%

0.0371%

0.0518%

0.0793%

0.4074%

PCR-High

0.0323%

0.0457%

0.0623%

0.1004%

0.3755%

RT-PCR

0.0368%

0.0526%

0.0793%

0.1403%

0.6571%

Real

0.0309%

0.0510%

0.0916%

0.2113%

100%

  1. This table contains the 25th, median 50th, 75th, 95th and the maximum 100th percentiles of genome position mismatch frequencies in each of the four samples.