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Table 3 Predicted terminators analysed by RT-PCR

From: Intrinsic terminators in Mycoplasma hyopneumoniae transcription

Terminator Validation 1 Localisation Class Gene 2 Distance (bp) 3 ΔG 4
w_053 No mC_15 tc3 pyrG 82 −5.01
wf_491 TE mC_28 tc4 pdhD-1 193 −4.51
wf_204 No mC_30 tc4 pulA 131 −2.48
w_202 No mC_31 tc4 dam 195 −4.69
wf_513 TE mC_33 tc4 gcp 190 −4.36
t_020 TE TU_010 tc2 nox 40 −6.5
wf_075 No TU_036 tc4 rpsT −9 −3.94
w_147 No TU_039 tc4 lip 181 −4.76
wf_375 TE TU_060 tc4 glpF 29 −2.22
wf_137 TE TU_066 tc4 metG 149 −3.21
w_076 No TU_085 tc2 pdhD 37 −9.01
t_264 TE TU_089 tc2 tuf 34 −7.5
wf_202 TE TU_098 tc4 fpg 52 −2.53
wf_215 TE TU_103 tc4 MHP7448_0621 198 −3.11
w_102 TE TU_113 tc2 MHP7448_0665 43 −11
  1. 1Presence of functional activity. TE — transcription termination; TD — transcription decreasing; No — without difference in transcript upstream and downstream of the terminator sequence.
  2. 2Gene at the end of the transcription unit.
  3. 3Distance between stop codon of the gene and the first nucleotide of terminator sequence.
  4. 4Value of free energy of Gibbs identified by the software that predicted the terminator.