Intrinsic terminators in Mycoplasma hyopneumoniae transcription

BMC Genomics

Table 3 Predicted terminators analysed by RT-PCR

Terminator	Validation ¹	Localisation	Class	Gene ²	Distance (bp) ³	ΔG ⁴
w_053	No	mC_15	t_c3	pyrG	82	−5.01
wf_491	TE	mC_28	t_c4	pdhD-1	193	−4.51
wf_204	No	mC_30	t_c4	pulA	131	−2.48
w_202	No	mC_31	t_c4	dam	195	−4.69
wf_513	TE	mC_33	t_c4	gcp	190	−4.36
t_020	TE	TU_010	t_c2	nox	40	−6.5
wf_075	No	TU_036	t_c4	rpsT	−9	−3.94
w_147	No	TU_039	t_c4	lip	181	−4.76
wf_375	TE	TU_060	t_c4	glpF	29	−2.22
wf_137	TE	TU_066	t_c4	metG	149	−3.21
w_076	No	TU_085	t_c2	pdhD	37	−9.01
t_264	TE	TU_089	t_c2	tuf	34	−7.5
wf_202	TE	TU_098	t_c4	fpg	52	−2.53
wf_215	TE	TU_103	t_c4	MHP7448_0621	198	−3.11
w_102	TE	TU_113	t_c2	MHP7448_0665	43	−11

¹Presence of functional activity. TE — transcription termination; TD — transcription decreasing; No — without difference in transcript upstream and downstream of the terminator sequence.
²Gene at the end of the transcription unit.
³Distance between stop codon of the gene and the first nucleotide of terminator sequence.
⁴Value of free energy of Gibbs identified by the software that predicted the terminator.

ISSN: 1471-2164