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Table 1 Comparison of precursors’ annotation results using the three approaches

From: Annotation of the goat genome using next generation sequencing of microRNA expressed by the lactating mammary gland: comparison of three approaches

  

De novo

Bovine

Goat

Potential precursors

Reads mapped (%)*

75.1

74.9

74.9

Total

1,327

1,017

1,067

Filtered** :

913

924

924

miRNA -5p and -3p

305

299

305

−5p + −3p annotated

172

172

172

−5p annotated + −3p putative

20

20

20

−5p putative + −3p annotated

14

13

14

−5p + −3p putative

99

94

99

Only -5p

298

303

305

−5p annotated

53

59

59

−5p putative

245

244

243

Only -3p

310

322

317

−3p annotated

60

67

66

−3p putative

250

255

251

Known precursors

Reads mapped (%)*

0

2.9

2

Total

1

808

267

Filtered**:

0

138

25

−5p + −3p known

0

4

8

Only -5p known

0

55

8

Only -3p known

0

49

8

−5p known + −3p annotated

0

17

0

−3p known + −5p annotated

0

13

1

  1. Annotated miRNA correspond to miRNA annotated by homology (perfect match full length) with mature miRNA from miRBase v21. Putative miRNA correspond to miRNA not yet described in any species. In bold, numbers correspond to the total of each subcategory.
  2. *Percentage of reads mapped on the set of potential precursors.
  3. **Only potential precursors with cumulative counts >10 have been retained.