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Table 1 Comparison of precursors’ annotation results using the three approaches

From: Annotation of the goat genome using next generation sequencing of microRNA expressed by the lactating mammary gland: comparison of three approaches

   De novo Bovine Goat
Potential precursors Reads mapped (%)* 75.1 74.9 74.9
Total 1,327 1,017 1,067
Filtered** : 913 924 924
miRNA -5p and -3p 305 299 305
−5p + −3p annotated 172 172 172
−5p annotated + −3p putative 20 20 20
−5p putative + −3p annotated 14 13 14
−5p + −3p putative 99 94 99
Only -5p 298 303 305
−5p annotated 53 59 59
−5p putative 245 244 243
Only -3p 310 322 317
−3p annotated 60 67 66
−3p putative 250 255 251
Known precursors Reads mapped (%)* 0 2.9 2
Total 1 808 267
Filtered**: 0 138 25
−5p + −3p known 0 4 8
Only -5p known 0 55 8
Only -3p known 0 49 8
−5p known + −3p annotated 0 17 0
−3p known + −5p annotated 0 13 1
  1. Annotated miRNA correspond to miRNA annotated by homology (perfect match full length) with mature miRNA from miRBase v21. Putative miRNA correspond to miRNA not yet described in any species. In bold, numbers correspond to the total of each subcategory.
  2. *Percentage of reads mapped on the set of potential precursors.
  3. **Only potential precursors with cumulative counts >10 have been retained.