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Figure 2 | BMC Genomics

Figure 2

From: An integrative approach to identify hexaploid wheat miRNAome associated with development and tolerance to abiotic stress

Figure 2

Overview of the predicted miRNAs. a) Diagram of the intersection between miRNAs predicted by the novel approach, conserved miRNAs identified by sequence homology, and miRNAs published in the literature; b) Evidence of conserved plant miRNA families present in miRBase including those predicted by our approach (tae, osa, bdi, hvu, ath and ptc correspond respectively to Triticum aestivum, Oryza sativa, Brachypodium distachyon, Hordeum vulgare L., Arabidopsis thaliana and Populus trichocarpa); c) The abundance bins of all predicted miRNAs in the 10 libraries (L1-L10) produced from plants grown under different investigated conditions. The abundance of the identified miRNAs represents the number of reads sequenced in each library and classified on 4 levels: low, 10–99 reads; medium, 100–999 reads; and high, 1000 and up; d) the length distribution of miRNAs associated with miRNAs differentially expressed in different investigated comparisons; e) the number of miRNA targeted genes (presented by EST id or UniRef id) associated with miRNAs differentially expressed in different investigated comparisons. WcvCl, winter wheat cultivar Clair (cold tolerant); ScvBo, spring wheat cultivar Bounty (cold and Al sensitive); WcvAt, winter wheat cultivar Atlas (Al tolerant); A.P, aerial parts; L./Rep. T., leaves and reproductive tissues; N.C, normal conditions; Al, Aluminum; Vern., vernalization; Rep., reproductive; Str. Resp., stress response; Tol., tolerance; Dev. resp., developmentally response; Fl. Trans, floral transition; Flw., flowering. See Additional file 1: Method S1 and Additional file 2: Table S1 for libraries and conditions and Additional file 2: Table S10 for the different investigated comparisons.

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