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Figure 4 | BMC Genomics

Figure 4

From: An integrative approach to identify hexaploid wheat miRNAome associated with development and tolerance to abiotic stress

Figure 4

Differentially expressed miRNAs in response to cold, salt, aluminum and development. a) The differential expression of miRNAs in response to vernalization (presented on log10 adjusted p-value based on the FDR method of Benjamini and Hochberg [80], associated with the log10 of the fold change (FC)). The lines specify the thresholds used to identify the most relevant differentially expressed miRNAs. The blue and red dots correspond respectively to expressed small RNAs and predicted miRNAs; b) the frequencies of differentially expressed miRNAs in response to vernalization, cold, Al, salt and development stage (floral transition and flowering); and those differentially expressed between tolerant and sensitive genotypes; c) Venn diagram of miRNAs regulated under short/long exposure to cold (cold/vernalization, L2/L1 and L7/L6) in leaves, Al (L10/L9 and L8/L9) in roots and salt (L4/L1) in leaves; d) Venn diagram of miRNAs expressed in control plants during vegetative phase under normal conditions (control library L1), plants acclimated up to 56 days at 4°C (vernalized library L2) during vegetative phase and, plants acclimated up to 56 days at 4°C and then transferred to normal conditions under long day photoperiod to induce flowering during the reproductive phase (reproductive library L3). Up, up-regulated miRNAs; Dw, down-regulated miRNAs; Cold/vrn, cold and vernalization responsive miRNAs in spring (L7/L6) and winter wheat (L7/L6), respectively; salt responsive miRNAs in winter wheat (L4/L1); Al responsive miRNAs in spring (L8/L9) and winter (L10/L9) wheat; For tolerance, only differentially expressed miRNAs between cold (L2/L7) and Al (L10/L8) treated libraries are presented. All other abbreviations’ are described in the legend of Figure 2. See Additional file 1: Method S1 and Additional file 2: Table S1 about libraries and conditions and Additional file 2: Table S9 for more information about regulated miRNAs.

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