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Figure 2 | BMC Genomics

Figure 2

From: Common binding by redundant group B Sox proteins is evolutionarily conserved in Drosophila

Figure 2

Results of comparative DamID for Dichaete and SoxN. (A) Dichaete-Dam binding profiles plotted on the D. melanogaster genome showing translated reads mapping to an orthologous region in four species. Three biological replicates from each species are shown. All read libraries were scaled to a total size of 1 million reads for visualization purposes, but were not normalised to the control. The y-axes of all tracks range from 0–50 reads. (B) SoxN-Dam binding profiles plotted on the D. melanogaster genome showing translated reads mapping to an orthologous region in two species. Three biological replicates from each species are shown. All read libraries were scaled to a total size of 1 million reads for visualization purposes, but were not normalised to the control. The y-axes of all tracks range from 0–50 reads. The same ~120-kb region of chromosome 2 L is shown in (A) and (B). (C) Overlaps between D. melanogaster Sox DamID binding intervals and known CRMs from REDFly and FlyLight at the decapentaplegic (dpp) locus. Binding profiles represent the normalised log2 fold changes between Sox fusion binding and Dam-only control binding in each GATC fragment. (D) De novo Sox motifs discovered in DamID binding intervals. Motifs discovered in Dichaete intervals in each species show a preference for T in the fourth position of the core CAAAG motif, while those discovered in SoxN intervals in each species show a preference for A. Abbreviations: mel, Drosophila melanogaster; sim, Drosophila simulans; yak, Drosophila yakuba; pse, Drosophila pseudoobscura.

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