Loss of γH2AX from the XY body in pachytene spermatocytes becomes evident at Stage VI of the spermatogenic cycle in the dog. A) Schematic drawing of the different stages of the spermatogenic cycle of the dog (adapted from ). B) Immunostaining of cryosections of dog testes for γH2AX (green) and SYCP3 (red), and also stained with DAPI (blue). Stages were identified based on Russell et al. (1990) . Loss of γH2AX and the thickened SYCP3 axes representative of late pachytene becomes evident at Stage VI. Single immunostainings are shown in grayscale. Magnifications are shown in the bottom panels, and some cells and the XY body are outlined for reference.