Figure 7

Different patterns of RNA polymerase II localization on the XY body of dog pachytene spermatocytes. A) Pachytene spermatocyte spread nuclei stained for γH2AX (blue), RNA polymerase II (RNApolII, red), and SYCP3 (green), single RNA polymerase II immunostainings are shown in gray scale. The lower panel represents a late pachytene spermatocyte in which γH2AX is already lost from part of the X chromosome and RNApolII is not clearly depleted from the XY body. B) Pachytene spermatocyte spread nuclei stained for γH2AX (blue), phosphorylated RNA polymerase II (RNApolII, red), and SYCP3 (green), single RNA polymerase II immunostainings are shown in gray scale. The lower panel represents a late pachytene spermatocyte in which γH2AX is already lost from part of the X chromosome. RNA polymerase II depletion from the XY body is evident in the nucleus shown in the middle. In addition, foci of phosphorylated RNA polymerase II staining are specifically observed in the XY body of the middle and the bottom nucleus. B’ shows magnifications of the XY body regions shown in B. C) Quantification of the percentages of pachytene nuclei (n = 101) with RNA polymerase II foci in the XY body, relative to the overall depletion of this mark from the (rest of ) the XY body, and to the enrichment of γH2AX in the XY body. Nuclei were immunostained as shown in panel B.