Figure 9
From: Incomplete meiotic sex chromosome inactivation in the domestic dog
Dynamics of H3K4me2 and H3K9me3 on the XY body in dog spermatocytes. A) Pachytene spermatocyte spread nuclei stained for DAPI (blue), H3.1/2 (red), H3K4me2 (white, artificial color chosen to represent the infrared signal) and SYCP3 (green). Single immunostainings are shown in grayscale. Subsequent substages indicated on the left are shown from top to bottom in the different panels. B) Spermatid spread nuclei stained for DAPI (blue), H3.1/2 (red), and H3K4me2 (white, artificial color chosen to represent the infrared signal). Single immunostainings are shown in grayscale. C) Pachytene spermatocyte spread nuclei stained for DAPI (blue), H3.1/2 (red), H3K9me3 (white, artificial color chosen to represent the infrared signal) and SYCP3 (green). Single immunostainings are shown in grayscale. Subsequent substages indicated on the left are shown from top to bottom in the different panels. D) Representative spermatid spread nuclei stained for DAPI (blue), H3.1/2 (red), and H3K9me3 (white, artificial color chosen to represent the infrared signal). Single immunostainings are shown in grayscale.