Identification and biological replication of sexually dimorphic genes identified in eight-cell embryos. Transcripts were defined as sex dimorphic from RNA-seq data using a genome-wide corrected p-value < 0.1 and −0.5 < log fold change (male/female) > 0.5 (A) Sexually dimorphic X-linked (51) and Y-linked (3) genes identified in the discovery set of six male and six female eight-cell embryos. (B) Autosomal genes (15) identified in the discovery set of six male and six female eight-cell embryos. Transcripts more highly expressed in a particular sex are indicated in red, those less expressed are indicated in blue. Rows representing individual transcripts were scaled to mean = 0, standard deviation = 1 (C) Average log fold change (male/female) for the discovery set (x-axis) plotted against log fold change (male/female) for an independent set of eight female and four male embryos (r = 0.95, p-value < 2.2 × 10−16). X-linked genes are indicated in red, Y-linked genes are indicated in blue and autosomal genes are indicated in green.