Validation of RNA-Seq library and analysis techniques. A. Life stage comparison of Cufflinks FPKM values to Sailfish RPKM values. Pearson’s correlation coefficient is represented for each life stage comparison. Genes used for comparison are those annotated in VectorBase release Agam3.7. B. Clustered FPKM expression (Additional file 1) of differentially expressed genes between life stages in An. gambiae. Rows and columns were clustered using Pearson correlation method with complete linkage distances. C. DAVID enrichment scores for differentially expressed gene groups between life stage comparisons.