Sequence, structural and expression profiles of identified gene classes. A. Characterization of sequence conservation across the genus Anopheles performed using PhyloP. The –log10(PhyloP Conservation P-Value) was calculated for each gene within each respective gene class and statistical significance was determined using a Mann–Whitney T-Test. Starred bars denote p-value <0.001. B. Stacked histogram of RNAz score output from REAPR analysis (delta value of 10) for lncRNA (red bars) and novel protein-coding genes (blue bars). Insert shows confident RNA secondary structure calls with an RNAz score above 0.5.