Variation in the vru upstream regions. Alignment of upstream region of vru gene across 13 strains was generated using Muscle . Boxshade server (http://www.ch.embnet.org/software/BOX_form.html) was used to highlight the high degree of conservation within the aligned region. The position of the initiating methionine codon (ATG) is shown with an arrow. Deletion of TATAA was found in isolates EF20, 6736 and 6780 in position -75 to -79 of vru gene. Polymorphism of A to T found in region -79 along with the deletion of four bases TATA in regions -75 to -78 of isolates B362 and C8329. Deletion of four bases ATAA (-76 to -79) was found in 3 isolates Ab71, C5388 and C9359.