Figure 2

The HTorA^ΔE-expressing flies had significantly increased HSC3 and HSP22 in their brains. (A and B) When the amounts of HSC3 were normalised with α-Tubulin as a loading control, the HTorA^ΔE-expressing brains had 1.4-fold more HSC3 than those of the HTorA^WT-expressing brains. However, Actin-enhanced Green fluorescence protein (Act-eGFP) expressing flies showed similar expression levels of HSC3. (C and D) In normal condition, HSP22 was detected from only the crude mitochondrial fraction of the HTorA^ΔE-expressing brains and it was 3.4-fold more abundant in the crude mitochondrial fraction of the HTorA^ΔE -expressing brains than that of the HTorA^WT-expressing brains before or following a heat shock. * = p < 0.05.