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Figure 4 | BMC Genomics

Figure 4

From: The pathogenic human Torsin A in Drosophila activates the unfolded protein response and increases susceptibility to oxidative stress

Figure 4

Increased sensitivities to oxidative and ER stressors and autophagy inhibitors in the HTorAΔE flies. (A) The hazard ratio (HR) of the HTorAΔE expressing flies reared on fly food containing 1% H2O2 was 2.073 times increased compared to those of the HTorAWT expressing flies. (B) The HTorAΔE-expressing flies also showed 3.564 times increased HR to 20 mM paraquat induced oxidative stress. (C) When ER stress was induced using 12 mM tunicamycin, the HR of the HTorAΔE-expressing flies was 3.013 times increased compared with the HTorAWT-expressing flies. (D-F) When a protein disulfide bond reducing compound DTT was applied with three different concentrations, including 5 mM (D), 25 mM (E), and 100 mM (F), there was no difference in HR between the HTorAWT and the HTorAΔE-expressing flies. (G-I) The HTorAΔE flies were vulnerable to autophagy inhibitors, 10 mM 3-MA (G), 40 μM Wortmannin (H), and 40 μM LY294002 (I) compared with the HTorAWT flies. HR = hazard ratio, 95% CI = 95% confident intervals of hazard ratio.

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