Table 3 Comparative statistics of assemblies generated from subsets of NaS or Illumina reads
From: Genome assembly using Nanopore-guided long and error-free DNA reads
Assembly 1 | Assembly 2 | Assembly 3 | Illumina_only assembly | |
|---|---|---|---|---|
MinION® coverage | 14.4x | 28.6x | 57.0x | NA |
Illumina | 50x @250 bp | 50x @250 bp | 50x @250 bp | 50x @250 bp |
NaS coverage | 5.3x | 10.6x | 23.0x | NA |
# of scaffolds | 19 | 5 | 1 | 20 |
Cumulative size (Mbp) | 3,431,926 | 3,599,306 | 3,600,135 | 3,592,537 |
Average size (bp) | 180,628 | 719,861 | 3,600,135 | 179,627 |
N50 size (bp) | 242,347 | 1,815,485 | 3,600,135 | 326,117 |
L50 | 4 | 1 | 1 | 5 |
N90 size (bp) | 83,428 | 421,811 | 3,600,135 | 140,386 |
L90 | 14 | 3 | 1 | 11 |
Max size (bp) | 755,415 | 1,815,485 | 3,600,135 | 520,993 |
Genome fraction (%) | 92.855 | 99.551 | 99.880 | 99.735 |
# misassemblies | 4 | 2 | 1 | 4 |
# mismatches per 100 kbp | 6.46 | 4.97 | 4.67 | 6.49 |
# indels per 100 kbp | 4.64 | 3.27 | 3.20 | 0.33 |