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Figure 4 | BMC Genomics

Figure 4

From: Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype

Figure 4

Gene replacement with the truncated GFP-XYR11–780 reproduced the XYR1-loss-of-function phenotype in QM9414. (A) QM9414::GFP-XYR11–780 transformants (TRAL007 clones 1 to 3) showed the same growth defect on 65 mM D-xylose as QM9136 and ∆xyr1, respectively, indicating loss of XYR1 function, which was further verified by the corresponding macroscopic colony phenotypes, shown in (B). (C) Gene expression of cellulase cel7a, xylanase xyn2 and xyr1 were not significantly upregulated in GFP-XYR11–780 expressing transformants (TRAL007), whereas the GFP-XYR1 transformants (TRAL002) clearly responded to the inductive stimulus provided by sophorose. Error bars show standard deviation (n = 6 from three experimental and two biological replicates involving two different TRAL007 clones).

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