Table 3 KASP assay primer sequences of three SNPs, GSM381, GSM383 and GSM191
Locus | Gene | Marker | Â |
|---|---|---|---|
Rhg1 | Gm18g02590 | GSM381 | FAM_primer: GAAGGTGACCAAGTTCATGCTAGCCAAAGAACTTGAGSAGBATGAG |
| Â | Â | Â | VIC_Primer: GAAGGTCGGAGTCAACGGATTAGCCAAAGAACTTGAGSAGBATGAT |
| Â | Â | Â | Common reverse primer: CAAACAATAGGTCCAACCACCA |
Rhg1 | Gm18g02590 | GSM383 | FAM_primer: GAAGGTGACCAAGTTCATGCTATCTGCMAACTCTGTAAAGAGGAC |
| Â | Â | Â | VIC_Primer: GAAGGTCGGAGTCAACGGATTATCTGCMAACTCTGTAAAGAGGAG |
| Â | Â | Â | Common reverse primer: GCTGTCCAGTCTCCAGAAGTGAA |
Rhg4 | Gm08g11490 | GSM191 | FAM_primer: GAAGGTGACCAAGTTCATGCTCATCATGGGGCTAGATCTCCC |
| Â | Â | Â | VIC_Primer: GAAGGTCGGAGTCAACGGATTCATCATGGGGCTAGATCTCCG |
| Â | Â | Â | Common reverse primer: TAGCCGGTGGTGGAGTTTACC |