Expression profiles of three predicted mRNA targets are inversely correlated with bpa-miR-5364. Graph shows levels of expression of Bm1_27305 (Ets-domain containing protein), Bm1_05425 (Zinc finger N-recognin family protein) and Bm1_25620 (high mobility group protein) in day 10 post-infective L4 compared to mosquito-derived L3. As B. pahangi material was used throughout, protein-coding genes with identifiers of the form “Bm1_00001” refer, here, to the B. pahangi orthologs of the B. malayi genes. The mean of three biological replicates is shown +/− standard deviation. Results were normalised to β tubulin and are expressed as log2 fold change relative to mosquito-derived (MD) L3.