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Table 1 Mean bpa-miR-5364 microarray signal in five B. pahangi life cycle stages

From: A novel member of the let-7 microRNA family is associated with developmental transitions in filarial nematode parasites

Reporter name (a)

Mosquito-derived L3

Day 1

Day 5

Day 10

Adult male

Adult female

bpa-let-7

93

214

2,713

3,068

2,678

1,398

bpa-miR-84-5p

4

18

409

669

1,134

758

bpa-miR-5364

708

8,794

14,922

17,301

9,173

8,518

cel-miR-789

808

2,165

58

48

213

26

bpa-miR-5853

1,329

2,079

636

275

933

207

  1. (a)Eight probes shown in Additional file 2 are not included here for the following reasons: cel-let-7, as its profile was essentially identical to bpa-let-7; bpa-miR-84-3p*, as it sequence is identical to bpa-miR-84-5p; Bpa0107, as its sequence corresponds to a tRNA [14]; bpa-miR-5853*, cel-miR-74 and bpa-miR-5872, due to low expression (defined as signal <500 for all replicates of the two time-points where expression was significantly different); cel-mir-51 and cel-mir-266, as the corresponding B. pahangi sequences could not be identified by PCR amplification of candidate sequences found from re-analysis of our previous small RNA deep sequence data [14].