Experimental design. The transcriptome study is consisted of wet lab procedures and data analysis processes. The parasite transcript sequences were analysed in terms of positive selection, cytokine signaling and pathogen database homology. The parasite sequences showing the signs of positive selection (Ka/Ks > 1) were termed PSRs (positive selection related transcripts). The cytokine signaling relation for the inferred parasite proteins were detected by the protein motif information and the related transcripts were termed CSRs (cytokine signaling related transcripts). Non-virulence-related transcripts (NVTs) were determined by the sequence homology of the parasite transcripts with the data of the non-pathogen associated databases (DDBJ, WormBase; E value < 10−5) by excluding the non-parasite organism homologous PSRs and CSRs. The parasite transcripts showing the exclusive sequence homology with the pathogen-associated databases (HSD and Violin) (E value < 10−5), but not being NVTs, were termed PRDs (pathogen database related transcripts). The parasite transcripts including PSRs, CSRs and PDRs were considered virulence-related transcripts (VRs). Immunomodulation categorisable VRs (PSRs/PDRs and all CSRs) were termed virulence and immunomodulation-related transcripts (VIRs). Asterisk (*) indicates that data from other resources (NCBI, UniProt, GeneDB, Gene Ontology and referred publications) were used for functional categorisation of some PSRs and PDRs.