Figure 2

Annotation of splice events in ERα + and ERα + ERβ + BC cell lines. (A) The bar plot shows the number of all alternative splicing events occurring in the cell lines analyzed. Inclusion and exclusion behavior for each event are shown (FDR ≤ 0.05; c ≤ |0.1|). (B) Genes whose regulation has opposite direction in the ERβ + lines compared to the wt MCF-7. The heat map on the right side shows the gene expression fold changes induced by estradiol. The matrix on the left side shows in black those genes for which a splicing event was detected in at least one of the cell lines. The nomenclature for the cell lines is the following: wt (parental MCF-7 cells); Ct-ERβ (MCF-7 subclone stably expressing ERβ tagged with the TAP-tag at the C-terminus); Nt-ERβ (MCF-7 subclone stably expressing ERβ tagged with the TAP-tag at the N-terminus).