Figure 8
The hexameric repeat/Alu-like domain modulates VNTR determined LAVA mobilization capacity. (A) Schematic representation of the LAVA_F chimeras tested. 5’ and 3’ domains of the two LAVA_F elements were reciprocally exchanged at the Alu-like/VNTR junction and/or the VNTR/3’ part junction. (B) Retrotransposition reporter assay following selection with G418. Cells were co-transfected with driver (pJM101 L1RP Δ Neo) and the respective mneoI-tagged chimeras or the LAVA_F full-length constructs. Retrotransposition rates (+/− standard deviation, n = 3) are given relative to that of the full-length LAVA_F1 construct (100%). (C) Repeat unit (RU) schemata of the two LAVA elements tested. RU arrays corresponding to the respective subfamily consensus are highlighted in grey.