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Table 2 Polymorphic changes in the genome of in vitro mutant strains

From: Mutations upstream of fabI in triclosan resistant Staphylococcus aureus strains are associated with elevated fabI gene expression

Ref. genome positions* Annotation RN4220 derived ATCC6538 derived Mu50 derived
NCTC8325 Mu50 MO034 MO035 MO036 d2** d7 MO052 MO079**
120767   Capsular polysaccharide synthesis enzyme Cap5B      A 592 T – Met 198 Leu   
208900   Pyruvate formate-lyase 1-activating enzyme       G 721 T - Val 241 Phe  
226813   Flavohemoprotein      -TA 953 deletion leading to transcription premature truncation   
784247   Protein traslocase subunit SecG      T 22 A – Leu 8 Ile   
919922   fabI upstream region**       T 109 G  
919929   fabI upstream region      A 101 deletion   
919959   fabI upstream region A 72 G       
919997   fabI upstream region   C 34 T      
920024   fabI upstream region    A 7 G     
920098   fabI coding region   G 68 C – Gly 23 Ala      
920331   fabI coding region G 301 T – Asp 101 Tyr   G 301 T – Asp 101 Tyr     
920469   fabI coding region      T 439 C – Tyr 147 His   
920641   fabI coding region     T 611 G – Phe 204 Cys   T 611 G – Phe 204 Cys  
  1060928 fabI coding region        T 611 A – Phe 204 Tyr
1139511   serine/threonine-protein kinase PrkC      G 1309 A – Val 437 Ile   
1231020   DNA mismatch repair protein MutL       T 1709 A – Ile 569 Asn  
  1238213 MurD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase        T 338 G – Ile 113 Ser
  1238872 MurD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase        C 997 G – Leu 333 Val
1588812   tRNA methylthiotransferase YqeV      G 824 A – Ile 275 Thr   
2335216   HTH-type transcriptional regulator SarV      C 140 A – Val 47 Gly   
2774594   Ica operon transcriptional regulator, IcaR      -TCTTTTGTCA 417 deletion leading to transcription premature truncation   
  1. *The nucleotide position refers to the genome of S. aureus NCTC8325 (accession NC_007795) or Mu50 (accession NC_002758). Polymorphic sites of fabI promoter region are indicated counting backwards from the sa-fabI start site of S. aureus Mu50 (GenBank ID: BA000017; position 1060308). **Despite not having fabI promoter mutations the d2 and MO079 mutants have been included in the analysis to check for the presence of mutations (not only in the fabI coding sequence) playing a possible role in triclosan reduced susceptibility.