Western blotting validation of proteome data. Protein was extracted from three biological replicates of control (C) and Z. tritici-inoculated (I) wheat cvs. Stakado (resistant) and Sevin (susceptible) at 5 and 14 dai and used in western blot analysis. The representative membranes of PR-1, PR-2 and PR-3 protein expression are shown. Quantification of signals on the membranes was performed by using ImageJ program based on three biological samples. The asterisks indicate significant differences in signal intensity (P ˂ 0.05) between control and inoculated samples.