Figure 3
Characterization of ΔZtYAP1 mutant. (A) ΔZtYAP1 mutant is hypersensitive to oxidants in vitro. Sensitivity of Z. tritici wild type (WT) and ΔZtYAP1 deletion strains D1 and D2 was determined by radial growth on PDA supplemented with oxidants or compounds as indicated. Results from one representative of two technical replicates are shown. (B) ΔZtYAP1 mutant is as virulent as WT in planta. Fungal pathogenicity was assayed on wheat leaves spray-inoculated with spore suspension (1 x 106 spores/mL) prepared from WT, D1, and D2 strains. Photos were taken at 10, 12 and 15 dai.