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Figure 2 | BMC Genomics

Figure 2

From: Fungal artificial chromosomes for mining of the fungal secondary metabolome

Figure 2

FAC transformants contain intact gene clusters confirmed by PCR. Panel a: Astechrome gene cluster (6J7) is present in A. nidulans transformed with FAC as examined by PCR. The seven astechrome genes (red) are not present in wild type A. nidulans but are in A. terreus and all A. nidulans 6J7 transformants. Panel b: Putative gene cluster 23 (9D19) is present in A. nidulans transformed with FAC 9D19 as examined by PCR. The seven cluster genes (red) are not present in wild type A. nidulans but are in A. terreus and A. nidulans 9D19 transformant. All of primers are listed in Additional file 2: Table S1. Panel c shows both Astechrome gene cluster (6J7) on the top and the cluster 23 (9D19) on the bottom.

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