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Figure 1 | BMC Genomics

Figure 1

From: Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex

Figure 1

Consolidated workflow for the identification of Paracoccidioides EST sequences harboring retrotransposons. All EST sequences (41,558) available at dbEST (release 110111) for download from Paracoccidioides species were used to build a local database. The clustered approach adopted (reciprocal BLAST and CAP3) resulted in 12,922 clusters, which were used for further similarity searches against different databases (NR of proteins from NCBI, Repbase and TEfam). Blast hit descriptions were analyzed based on a lexical search approach (for details see Methods) and transposons matching rules suggested by Wicker and coworkers [2] for classifying eukaryotic TEs were adopted. A set of 52 EST sequences were found to be common between the results from similarity and lexical searches.

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