Figure 3

PCR screening for the reverse transcriptase domain of RtPc elements in phylogenetic lineages of Paracoccidioides species. Electropherogram results from PCR analysis of a phylogenetically diverse panel of Paracoccidioides species. PCR amplification of the reverse transcriptase domain of the five RtPc elements from the genomic DNA of 31 isolates of the three cryptic species of P. brasiliensis and three isolates of P. lutzii. Panel A shows the PCR amplification products for the five RtPc elements from the genomic DNA of the three isolates sequenced in the Broad Institute FGI (Pb01, P03 and Pb18). Panel B shows the amplification products for the 5 RtPc elements from the genomic DNA of 31 Paracoccidioides isolates. The first block is composed by two P. lutzii isolates. The second block by four P. brasiliensis, the first two being PS2 and the latter two PS3 isolates. In the third block the first 13 isolates are P. brasiliensis S1 and the latter four are Paracoccidioides spp. The fourth block, at right, is composed by eight Paracoccidioides spp. Oligonucleotide primer pairs are given in Additional file 6.