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Table 1 Summary Statistics for individual and merged assemblies

From: Transcriptome sequencing and annotation of the polychaete Hermodice carunculata (Annelida, Amphinomidae)

Assembly Number of transcripts > 200 bp N50 bp Mean length bp Max length bp Total number of bp
K-mer 21 143,191 584 505.54 7,342 72,390,913
K-mer 25 160,583 771 605.87 13,382 97,292,569
K-mer 29 188,890 631 523.05 8,878 98,798,757
K-mer 35 225,756 689 551.61 11,724 124,529,844
K-mer 41 179,143 891 633.86 18,825 113,522,250
K-mer 45 171,154 983 667.66 24,711 114,273,429
K-mer 51 156,387 1,096 713.03 17,800 111,509,378
K-mer 55 144,565 1,160 740.32 14,922 107,023,822
Final 525,989 1,095 722.3 24,711 379,922,870
Generated ORFs from Assembly Number of ORFs >200 AA N50 AA Mean length AA Max length AA Total number of AA
ORFs > 200AA 58,454 490 443.92 8,167 25,948,636
  1. For each k-mer, data from AbySS is shown. The final assmbly is the result of merging the AbySS k-mer assemblies using BLAT to remove the redundancies. Predicted ORF’s longer than 200AA’s from this final contig set were used for annotation. K-mer = required length of overlap match between two reads in AbySS; N50 = length weigthed median contig length; bp = base pair; ORF = Open Reading Frame.