Alignment of NifQ seuquences. The NifQ protein sequences of the ten sequenced N. galegae strains were aligned to those of the model system species A. vinelandii and K. pneumoniae, and the rhizobial relatives Sinorhizobium fredii and Rhizobium tropici. The last two amino acid residues of S. fredii are not visible in the figure. Bottom line: Amino acid residues that are conserved in all N. galegae strains are indicated with “^”, residues conserved in all included rhizobial strains with “!” and residues conserved in all strains in the alignment are indicated with “#”. The molybdenum-binding motif region (Cx4Cx2Cx5C ), indicated with a yellow box, has not been preserved in N. galegae.