Table 6 Primers used in this study
From: Genomic features separating ten strains of Neorhizobium galegae with different symbiotic phenotypes
Primer | Sequence 5′-3′ |
|---|---|
PCR amplification | Â |
RpoNLLSpeI | TTTAAAACTAGTTTGATATCGTCACCAATGGC |
RpoNLRBamHI | AAATTTGGATCCGTTTTCATTCAGAGCTCCTG |
nifQLLSpeI-ori | TTTAAAACTAGTCGATCGTCGTGAGCGCAATG |
nifQLRBamHI-ori | AAATTTGGATCCAGTTTCCATCCTGCGACCTC |
nifQLLSpeI-2 | AAATTTACTAGTGCTGCATCTCGGGAGGTCGAT |
nifQLRBamHI-2 | TTTAAAGGATCCAGCCATCTCGCAATTCACAGCG |
RpoNRLBglII | AAATTTAGATCTTTCTGACTCTGTCATGCTTC |
RpoNRRXhoI | TTTAAACTCGAGGTCCATAGTAAGTGCCGTGA |
nifQRLBamHI | AAATTTGGATCCCGACAATGATTGCGAATCAAGC |
nifQRRXhoI | TTTAAACTCGAGCGGCCGAACCCTTTGAACTC |
rpoNmutLL | AAACAAGATCATGCCATCGG |
540nifQL-1234F | TAGTCCGCCTGAGTCCAGAG |
1141nifQL-1184F | GCTTGTGATTGACGAGGTG |
hsnTmutLR | ACTATCAGGTCAAGTCTGCT |
hsnTmutRL | TTGATGTTACCCGAGAGCTT |
rpoNmutRR | CTTCATCCGATCCTGGAGTA |
nifQR-1209R | TCGCTATTGCATTGCCGATT |
Sequencing | Â |
rpoNL-646 | TGCAGTACAGCGATTGTGACG |
oriNifQL-570 | TGACGCAAGCAGTCGGCAAC |
offNifQL-661 | CATGGCAACGATGGCAAGTC |
rpoNR-413 | GCAACCGCAACGTGTATTCG |
oriNifQR-583 | GGATATTGCCATCCGCGAAG |
offNifQR-560 | TACCGAGATGAACAGCACCTG |