Transcriptome assembly quality assessment. A) Transcript length distribution. B) Number of transcripts per gene component as determined by Trinity. C) Top species hits from BLAST against NCBI’s nr database, first nine species are teleost fish sequences out of a total of 17 teleost species within the list, indicated by *. D) Quantitative PCR (qPCR) was used to verify transcriptome-derived FPKM values of 19 genes in SNH and SMH tissue groups. Pearson’s correlation analysis showed that qPCR and transcriptome measured ratios of SNH/SMH are significantly correlated with each other.