Detailed cytogenetic map of RH chromosome 5. This map was created by simultaneous fluorescence in situ hybridization of 35 BAC clones from the sequence MTPs to a pachytene chromosome spread. Intense DAPI staining of the condensed DNA of the pericentromeric heterochromatin is visible as white background to the colored BAC fluorescence signals. An asterisk marks the centromeric constriction. Subtelomeric BAC clone RH042N03 (yellow) produced a second fluorescence signal at the south end of Chr-5 (labelled in brackets). Eighty three percent of clone RH042N03 consists of the CL14 subtelomeric repeat [73,74] and this ectopic hybridisation signal most likely is caused by the presence of a similar subtelomeric repeat at the south terminus of Chr-5. Tiling path and marker data for the hybridized BACs are listed in Table 4.