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Figure 5 | BMC Genomics

Figure 5

From: Characterization of repetitive DNA landscape in wheat homeologous group 4 chromosomes

Figure 5

Physical localization of newly identified transposons. The identified retrotransposons were localized on metaphase spreads of vc. Chinese Spring using in situ hybridization with fluorescent labeled probes. Red color was used for the repetitive probes, Green color was used for Afa probe and Blue was stained chromosome DNA using DAPI. Arrows are pointing at 4D chromosome. a) Probes of all retrotransposons besides retrotransposon Carmen produced weak disperse signals on all chromosomes. The red signals represent distribution of retrotransposon Victoria. b) The red channel of figure A to demonstrate distribution of weak signals of Victoria probe on whole chromosomes. c) All probes derived from retrotransposon Carmen gave similar hybridisation pattern on metaphase chromosomes. Surprisingly, the retrotransposon was preferentially localized in centromeric region of all chromosomes with varying intensity. On few chromosomes were observed weak and dispersed signals on distal parts of chromosome arms too. d) The red channel of figure C to demonstrate distribution of weak signals of Carmen probe on whole chromosomes. Scale bars represent 10 μm.

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